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KMID : 0364820130490010064
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Korean Journal of Microbiology
2013 Volume.49 No. 1 p.64 ~ p.70
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Characterization of Entomopathogenic Fungus from Trialeurodes vaporariorum and Evaluation as Insecticide
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Yun Hwi-Geon
Shin Tae-Young
Yu Mi-Ra
Lee Won-Woo
Ko Seung-Hyun
Bae Sung-Min
Choi Jae-Bang
Woo Soo-Dong
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Abstract
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The greenhouse whitefly, Trialeurodes vaporariorum, is an economically important pest for greenhouse crops
because they cause direct damage by feeding on plant nutrients and indirect damage as transmits many virus vectors.
It has recently become a serious problem because of the continuous use of insecticide resulting in resistance among
greenhouse whitefly population. To overcome these problems, in this study, the biological characteristics and
virulence of an entomopathogenic fungus isolated from the cadaver of nymph greenhouse whitefly were
investigated. Isolated fungus was identified as Isaria fumosorosea by morphological examinations and genetic
identification using sequences of the ITS, β-tubulin, and EF1-α regions. This fungus was named as I. fumosorosea
SDTv and tested for the virulence against nymphs T. vaporariorum and the cold activity, the thermotolerance and the
stability of UV-B irradiation on conidia. Mortality rate of greenhouse whitefly showed from 84 to 100% and the
virulence increased with increasing conidial concentrations, 1×105
to 108
conidia/ml. Conidia were stable at 35℃,
0.1 J/cm2 of UV irradiation and germinated after 8 days at 4℃. Additionally, the activities of chitinases and proteases
produced by I. fumosorosea SDTv were varied according to the medium. In conclusion, I. fumosorosea SDTv which
showed high mortality rate against greenhouse whitefly will be used effectively in the integrated pest management
programs against the greenhouse whitefly.
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KEYWORD
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Isaria fumosorosea, Trialeurodes vaporariorum, entomopathogenic fungus, fungal insecticide
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